You are here

Past Project 2016-17: Effect of 2,4-thiazolidinedione on goat response to mastitis and milk fat - Study two

The objectives of the study were to assess in well-fed lactating goats the effect of 2,4-thiazolidinedione, a putative PPARgamma agonist, on: 1) milk fat production 2) the response to induced sub-clinical mastitis

Main findings.

Objective 1 - We determined that the daily injection of 8 mg/kg body weight of 2,4-thiazolidinedione in well-fed Saanen dairy goats dramatically reduced the NEFA and the BHBA in blood and increased glucose with consequent increase in adipocytes size. It affected also the expression of insulin- and glucose metabolism-related genes in the adipose tissue but had minor effect on mammary cells.

Objective 2 - The injection of 2,4-thiazolidinedione slightly improved the liver situation but did not affect the inflammatory response of mammary epithelial cells or macrophages isolated from milk. Data also indicated that 2,4-thiazolidinedione is not a PPARgamma agonist. 

Overall well-fed goats had a lower response to 2,4-thiazolidinedione compared to the same goats in less than optimal nutritional status.

Peer-Reviewed Publication:

Jaaf S, Rosa F, Moridi M, Osorio JS, Lohakare J, Trevisi E, Filley S, Cherian G, Estill CT, Bionaz M. 2,4-Thiazolidinedione in Well-Fed Lactating Dairy Goats: I. Effect on Adiposity and Milk Fat Synthesis. Vet Sci. 2019 May 17;6(2):45. doi: 10.3390/vetsci6020045. PMID: 31108904; PMCID: PMC6632146.


Conference abstracts


Jaaf S., Rosa F., Moridi, M., J. Osorio, Lohakare J., Trevisis E., Estill C., Cherian G., and M. Bionaz. 2018. Effect of 2,4-thiazolidinedione treatment on milk fat synthesis in lactating dairy goats in optimal nutritional status. 2018 ADSA Annual Meeting

Tsai C. Y., F. Rosa, M. Bionaz, and P. Rezamand. 2017. Effects of 2, 4- Thiazolidinedione on milk fatty acid profile and vitamins in dairy goat with subclinical mastitis. Anim. 2018 ADSA Annual Meeting


Rosa F., Moridi, M., J. Osorio, Lohakare, J. Estill C., and M. Bionaz. Macrophage activation during subclinical mastitis in dairy goats treated with 2,4-thiazolidinedione 2017 ADSA Annual Meeting

Tsai C. Y., F. Rosa, M. Bionaz, and P. Rezamand. 2017. The effect of 2,4- thiazolidinedione on lipid soluble vitamins in lactating goats induced with sub-clinical mastitis. Anim. 2017 ADSA Annual Meeting


Rosa F., J. S. Osorio, J. Lohakare, M. Moridi, A. Ferrari, E. Trevisi, and M. Bionaz. 2016 Percentages of milk fat, lactose, and protein are affected by diurnal variations in dairy goats. J. Anim. Sci Vol. 94, E-Suppl. 5: 410

Rosa F., M. Moridi, J. S. Osorio, J. Lohakare, S. Filley, J. L. Belveal, J. J. Bruton, E. Trevisi, C. Estill, and M. Bionaz. 2016 Effect of 2,4-thiazolidinedione treatment in the infammatory response to induced subclinical mastitis in dairy goats receiving adequate vitamin supplementation. J. Anim. Sci Vol. 94, E-Suppl. 5: 341

Project members: 
Graduate Student
Graduate Student
Principle Investigator
Principle Investigator

Oregon State University

CorvallisBenton County, Oregon 97331
United States
Latitude: 44.563781
Longitude: -123.279444

Funding Source

Oregon Beef Council  


All Labs