It is hypothesized that bovine cells will show a reduction in oxidative stress and an increase in viability when Nrf2 is activated in an in vitro model. It is also hypothesized that cows grazing legume (containing birdsfoot trefoil) and forb pastures (containing chicory and plantain) will have greater Nrf2 activation and lower oxidative stress than the cows fed TMR or grazing grass-clover pastures that contain less polyphenols.
Objectives are: 1) Assess the antioxidant role of Nrf2 in bovine cells using advanced molecular biology techniques and 2) Determine the effect of feeding cows with forages that contain different types and amounts of secondary metabolites on the antioxidant status of the cows and on the activation of Nrf2 using an in vitro-in vivo hybrid approach
Co-PIs in the proposal are Hunter Ford and Dr. Serkan Ates
This is the main project for Hunter Ford.
The project is supported by the Agricultural Research Foundation
- Ford Hunter R., Busato Sebastiano, Bionaz Massimo. In vitro–In vivo Hybrid Approach for Studying Modulation of NRF2 in Immortalized Bovine Mammary Cells. Front. Anim. Sci., 21 June 2021 | https://doi.org/10.3389/fanim.2021.674355
- Hunter Ford, Sebastiano Busato, Erminio Trevisi, Ruth N. Muchiri, Richard B. Van Breemen, Massimo Bionaz and Serkan Ates. Effects of pasture type on metabolism, liver and kidney function, antioxidant status, and plant secondary compounds in plasma of grazing, Jersey dairy cattle during mid-lactation. Front. Anim. Sci. | doi: 10.3389/fanim.2021.729423