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Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue.

TitleMyogenic potential of mesenchymal stem cells isolated from porcine adipose tissue.
Publication TypeJournal Article
Year of Publication2018
AuthorsMilner, DJ, Bionaz, M, Monaco, E, Cameron, JAnn, Wheeler, MB
JournalCell Tissue Res
Date Published2018 Jan 09

Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine CCmyoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with CCcells resulted in GFPmyotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFPASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.

Alternate JournalCell Tissue Res.
PubMed ID29318389
Grant List#2007-04072 / / Carle Foundation Hospital / United States
IDPH # 63080017 / / Illinois Regenerative Medicine Institute / United States